Sr .NO

Parameter

Condition used for analysis

1

Mobile phase

Tetra butyl Ammonium hydroxide solution (0.1 N aqueous ) pH 6.5 with orthophospharic acid (10% aqueous): Acetonitrile (3:1)

2

Flow rate

1.0 ml/min

3

Detection Wavelength

210 nm

4

Sample injector

50 µl loop

5

Column

C18 waters (4.6 × 250 mm, 5 µm)