Sr .NO | Parameter | Condition used for analysis |
1 | Mobile phase | Tetra butyl Ammonium hydroxide solution (0.1 N aqueous ) pH 6.5 with orthophospharic acid (10% aqueous): Acetonitrile (3:1) |
2 | Flow rate | 1.0 ml/min |
3 | Detection Wavelength | 210 nm |
4 | Sample injector | 50 µl loop |
5 | Column | C18 waters (4.6 × 250 mm, 5 µm) |